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mouse t testosterone elisa kit  (Wuhan Fine Biotech)

 
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    Wuhan Fine Biotech mouse t testosterone elisa kit
    Genomic instability is observed in InCl 3 -treated TM3 cells. InCl 3 induces enlarged nuclei and micronuclei (as indicated by white arrowheads) in TM3 cells. (A) Nuclear shapes of CTL and InCl 3 -treated TM3 cells were analyzed using DAPI staining. The right panel represents enlarged views of selected regions. Micronuclei, small DAPI staining near the nucleus, are indicated by the arrowheads. Mitotic cells were identified and are marked with red circles. Scale bar, 10 µm. (B) Quantitative results of the nuclear area shown through DAPI staining. (C) Quantification of the proportions of cells with micronuclei [as indicated by selected regions 1 and 2 of (A), right panel]. (D) InCl 3 treatment did not affect the mitotic index. The proportions of mitotic cells (mitotic index) were quantified. (E) InCl 3 treatment reduced the levels of <t>testosterone</t> in the culture medium of TM3 cells. Quantitative results of relative testosterone levels measured through <t>ELISA</t> in the absence or presence of InCl 3 . InCl 3 treatment reduced steroidogenic gene expression. (F) Schematic diagram of testosterone production. Quantitative results of relative mRNA levels of (G) StAR, (H) HSD3B and (I) CYP11A1. Results are presented as mean ± SD from ≥3 independent experiments. *P<0.05, **P<0.01 and ***P<0.001. n.s., not significant; InCl 3 , indium chloride; StAR, steroidogenic acute regulatory protein; HSD3B, 3-β-hydroxy-δ-steroid dehydrogenase; CYP11A1, cytochrome P450 family 11 subfamily A member 1; E, enlarged nuclei; CTL, control.
    Mouse T Testosterone Elisa Kit, supplied by Wuhan Fine Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+testosterone+elisa+kit/elisa+kit+mouse+testosterone/pmc13148121-108-10-18
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    Images

    1) Product Images from "Indium (III) chloride inhibits testicular Leydig cell proliferation by disrupting centrosome copy numbers"

    Article Title: Indium (III) chloride inhibits testicular Leydig cell proliferation by disrupting centrosome copy numbers

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2026.13891

    Genomic instability is observed in InCl 3 -treated TM3 cells. InCl 3 induces enlarged nuclei and micronuclei (as indicated by white arrowheads) in TM3 cells. (A) Nuclear shapes of CTL and InCl 3 -treated TM3 cells were analyzed using DAPI staining. The right panel represents enlarged views of selected regions. Micronuclei, small DAPI staining near the nucleus, are indicated by the arrowheads. Mitotic cells were identified and are marked with red circles. Scale bar, 10 µm. (B) Quantitative results of the nuclear area shown through DAPI staining. (C) Quantification of the proportions of cells with micronuclei [as indicated by selected regions 1 and 2 of (A), right panel]. (D) InCl 3 treatment did not affect the mitotic index. The proportions of mitotic cells (mitotic index) were quantified. (E) InCl 3 treatment reduced the levels of testosterone in the culture medium of TM3 cells. Quantitative results of relative testosterone levels measured through ELISA in the absence or presence of InCl 3 . InCl 3 treatment reduced steroidogenic gene expression. (F) Schematic diagram of testosterone production. Quantitative results of relative mRNA levels of (G) StAR, (H) HSD3B and (I) CYP11A1. Results are presented as mean ± SD from ≥3 independent experiments. *P<0.05, **P<0.01 and ***P<0.001. n.s., not significant; InCl 3 , indium chloride; StAR, steroidogenic acute regulatory protein; HSD3B, 3-β-hydroxy-δ-steroid dehydrogenase; CYP11A1, cytochrome P450 family 11 subfamily A member 1; E, enlarged nuclei; CTL, control.
    Figure Legend Snippet: Genomic instability is observed in InCl 3 -treated TM3 cells. InCl 3 induces enlarged nuclei and micronuclei (as indicated by white arrowheads) in TM3 cells. (A) Nuclear shapes of CTL and InCl 3 -treated TM3 cells were analyzed using DAPI staining. The right panel represents enlarged views of selected regions. Micronuclei, small DAPI staining near the nucleus, are indicated by the arrowheads. Mitotic cells were identified and are marked with red circles. Scale bar, 10 µm. (B) Quantitative results of the nuclear area shown through DAPI staining. (C) Quantification of the proportions of cells with micronuclei [as indicated by selected regions 1 and 2 of (A), right panel]. (D) InCl 3 treatment did not affect the mitotic index. The proportions of mitotic cells (mitotic index) were quantified. (E) InCl 3 treatment reduced the levels of testosterone in the culture medium of TM3 cells. Quantitative results of relative testosterone levels measured through ELISA in the absence or presence of InCl 3 . InCl 3 treatment reduced steroidogenic gene expression. (F) Schematic diagram of testosterone production. Quantitative results of relative mRNA levels of (G) StAR, (H) HSD3B and (I) CYP11A1. Results are presented as mean ± SD from ≥3 independent experiments. *P<0.05, **P<0.01 and ***P<0.001. n.s., not significant; InCl 3 , indium chloride; StAR, steroidogenic acute regulatory protein; HSD3B, 3-β-hydroxy-δ-steroid dehydrogenase; CYP11A1, cytochrome P450 family 11 subfamily A member 1; E, enlarged nuclei; CTL, control.

    Techniques Used: Staining, Enzyme-linked Immunosorbent Assay, Gene Expression, Control

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    Article Title: Targeting cancer stress-associated hyperinsulinemia and abnormal behavior mitigates lung carcinoma in postmenopausal mouse: Intervention function of peimine
    Article Snippet: The serum levels of luteinizing hormone (LH) were measured using an enzyme-linked immunosorbent assay (ELISA) kit (#ml063366, Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China) following the instructions provided by the manufacturer. .. The Mouse Testosterone ELISA Kit (#EM1850, Wuhan Fine Biotech Co., Ltd., Wuhan, China) was used to quantify serum testosterone levels following the instructions provided by the manufacturer. .. Progesterone levels were quantified using an enzyme-linked immunosorbent assay (ELISA) kit (#ab108670, Abcam) according to the manufacturer’s instructions.

    Article Title: Expression of asprosin and OLFR734 in reproductive tissues of polycystic ovary syndrome mice: insights into metabolic and reproductive dysfunction.
    Article Snippet: Asprosin, a fasting-induced adipokine, has been reported to exhibit altered circulating levels in women with polycystic ovary syndrome (PCOS); however, its tissue-specific regulation and the role of its receptor, OLFR734, in PCOS remain poorly understood.. In this study, we present the first histological evidence of asprosin and OLFR734 expression in reproductive tissues using dehydroepiandrosterone-induced PCOS mouse models maintained on chow or a high-fat diet (HFD).. Metabolic profiling revealed distinct phenotypes, with HFD-fed PCOS mice showing pronounced metabolic disturbances, including increased body weight, glucose intolerance, dyslipidemia, and elevated serum asprosin levels, compared with chow-fed PCOS mice.

    Article Title: Targeting SIRT3 signaling alleviates lung carcinoma progression by reducing hyperinsulinemia in postmenopausal obese mice: Protective intervention of betaxanthin
    Article Snippet: The serum levels of luteinizing hormone (LH) were determined using an enzyme-linked immunosorbent assay (ELISA) kit (#ml063366, Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China) according to the manufacturer’s instructions. .. The Mouse Testosterone ELISA Kit (#EM1850, Wuhan Fine Biotech Co., Ltd., Wuhan, China) was utilized to measure serum testosterone levels in accordance with the instructions provided by the provider. .. Progesterone levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (#ab108670, Abcam) following the directions provided by the manufacturer.



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    Genomic instability is observed in InCl 3 -treated TM3 cells. InCl 3 induces enlarged nuclei and micronuclei (as indicated by white arrowheads) in TM3 cells. (A) Nuclear shapes of CTL and InCl 3 -treated TM3 cells were analyzed using DAPI staining. The right panel represents enlarged views of selected regions. Micronuclei, small DAPI staining near the nucleus, are indicated by the arrowheads. Mitotic cells were identified and are marked with red circles. Scale bar, 10 µm. (B) Quantitative results of the nuclear area shown through DAPI staining. (C) Quantification of the proportions of cells with micronuclei [as indicated by selected regions 1 and 2 of (A), right panel]. (D) InCl 3 treatment did not affect the mitotic index. The proportions of mitotic cells (mitotic index) were quantified. (E) InCl 3 treatment reduced the levels of <t>testosterone</t> in the culture medium of TM3 cells. Quantitative results of relative testosterone levels measured through <t>ELISA</t> in the absence or presence of InCl 3 . InCl 3 treatment reduced steroidogenic gene expression. (F) Schematic diagram of testosterone production. Quantitative results of relative mRNA levels of (G) StAR, (H) HSD3B and (I) CYP11A1. Results are presented as mean ± SD from ≥3 independent experiments. *P<0.05, **P<0.01 and ***P<0.001. n.s., not significant; InCl 3 , indium chloride; StAR, steroidogenic acute regulatory protein; HSD3B, 3-β-hydroxy-δ-steroid dehydrogenase; CYP11A1, cytochrome P450 family 11 subfamily A member 1; E, enlarged nuclei; CTL, control.
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    Genomic instability is observed in InCl 3 -treated TM3 cells. InCl 3 induces enlarged nuclei and micronuclei (as indicated by white arrowheads) in TM3 cells. (A) Nuclear shapes of CTL and InCl 3 -treated TM3 cells were analyzed using DAPI staining. The right panel represents enlarged views of selected regions. Micronuclei, small DAPI staining near the nucleus, are indicated by the arrowheads. Mitotic cells were identified and are marked with red circles. Scale bar, 10 µm. (B) Quantitative results of the nuclear area shown through DAPI staining. (C) Quantification of the proportions of cells with micronuclei [as indicated by selected regions 1 and 2 of (A), right panel]. (D) InCl 3 treatment did not affect the mitotic index. The proportions of mitotic cells (mitotic index) were quantified. (E) InCl 3 treatment reduced the levels of <t>testosterone</t> in the culture medium of TM3 cells. Quantitative results of relative testosterone levels measured through <t>ELISA</t> in the absence or presence of InCl 3 . InCl 3 treatment reduced steroidogenic gene expression. (F) Schematic diagram of testosterone production. Quantitative results of relative mRNA levels of (G) StAR, (H) HSD3B and (I) CYP11A1. Results are presented as mean ± SD from ≥3 independent experiments. *P<0.05, **P<0.01 and ***P<0.001. n.s., not significant; InCl 3 , indium chloride; StAR, steroidogenic acute regulatory protein; HSD3B, 3-β-hydroxy-δ-steroid dehydrogenase; CYP11A1, cytochrome P450 family 11 subfamily A member 1; E, enlarged nuclei; CTL, control.
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    Genomic instability is observed in InCl 3 -treated TM3 cells. InCl 3 induces enlarged nuclei and micronuclei (as indicated by white arrowheads) in TM3 cells. (A) Nuclear shapes of CTL and InCl 3 -treated TM3 cells were analyzed using DAPI staining. The right panel represents enlarged views of selected regions. Micronuclei, small DAPI staining near the nucleus, are indicated by the arrowheads. Mitotic cells were identified and are marked with red circles. Scale bar, 10 µm. (B) Quantitative results of the nuclear area shown through DAPI staining. (C) Quantification of the proportions of cells with micronuclei [as indicated by selected regions 1 and 2 of (A), right panel]. (D) InCl 3 treatment did not affect the mitotic index. The proportions of mitotic cells (mitotic index) were quantified. (E) InCl 3 treatment reduced the levels of <t>testosterone</t> in the culture medium of TM3 cells. Quantitative results of relative testosterone levels measured through <t>ELISA</t> in the absence or presence of InCl 3 . InCl 3 treatment reduced steroidogenic gene expression. (F) Schematic diagram of testosterone production. Quantitative results of relative mRNA levels of (G) StAR, (H) HSD3B and (I) CYP11A1. Results are presented as mean ± SD from ≥3 independent experiments. *P<0.05, **P<0.01 and ***P<0.001. n.s., not significant; InCl 3 , indium chloride; StAR, steroidogenic acute regulatory protein; HSD3B, 3-β-hydroxy-δ-steroid dehydrogenase; CYP11A1, cytochrome P450 family 11 subfamily A member 1; E, enlarged nuclei; CTL, control.
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    Genomic instability is observed in InCl 3 -treated TM3 cells. InCl 3 induces enlarged nuclei and micronuclei (as indicated by white arrowheads) in TM3 cells. (A) Nuclear shapes of CTL and InCl 3 -treated TM3 cells were analyzed using DAPI staining. The right panel represents enlarged views of selected regions. Micronuclei, small DAPI staining near the nucleus, are indicated by the arrowheads. Mitotic cells were identified and are marked with red circles. Scale bar, 10 µm. (B) Quantitative results of the nuclear area shown through DAPI staining. (C) Quantification of the proportions of cells with micronuclei [as indicated by selected regions 1 and 2 of (A), right panel]. (D) InCl 3 treatment did not affect the mitotic index. The proportions of mitotic cells (mitotic index) were quantified. (E) InCl 3 treatment reduced the levels of <t>testosterone</t> in the culture medium of TM3 cells. Quantitative results of relative testosterone levels measured through <t>ELISA</t> in the absence or presence of InCl 3 . InCl 3 treatment reduced steroidogenic gene expression. (F) Schematic diagram of testosterone production. Quantitative results of relative mRNA levels of (G) StAR, (H) HSD3B and (I) CYP11A1. Results are presented as mean ± SD from ≥3 independent experiments. *P<0.05, **P<0.01 and ***P<0.001. n.s., not significant; InCl 3 , indium chloride; StAR, steroidogenic acute regulatory protein; HSD3B, 3-β-hydroxy-δ-steroid dehydrogenase; CYP11A1, cytochrome P450 family 11 subfamily A member 1; E, enlarged nuclei; CTL, control.
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    Image Search Results


    Genomic instability is observed in InCl 3 -treated TM3 cells. InCl 3 induces enlarged nuclei and micronuclei (as indicated by white arrowheads) in TM3 cells. (A) Nuclear shapes of CTL and InCl 3 -treated TM3 cells were analyzed using DAPI staining. The right panel represents enlarged views of selected regions. Micronuclei, small DAPI staining near the nucleus, are indicated by the arrowheads. Mitotic cells were identified and are marked with red circles. Scale bar, 10 µm. (B) Quantitative results of the nuclear area shown through DAPI staining. (C) Quantification of the proportions of cells with micronuclei [as indicated by selected regions 1 and 2 of (A), right panel]. (D) InCl 3 treatment did not affect the mitotic index. The proportions of mitotic cells (mitotic index) were quantified. (E) InCl 3 treatment reduced the levels of testosterone in the culture medium of TM3 cells. Quantitative results of relative testosterone levels measured through ELISA in the absence or presence of InCl 3 . InCl 3 treatment reduced steroidogenic gene expression. (F) Schematic diagram of testosterone production. Quantitative results of relative mRNA levels of (G) StAR, (H) HSD3B and (I) CYP11A1. Results are presented as mean ± SD from ≥3 independent experiments. *P<0.05, **P<0.01 and ***P<0.001. n.s., not significant; InCl 3 , indium chloride; StAR, steroidogenic acute regulatory protein; HSD3B, 3-β-hydroxy-δ-steroid dehydrogenase; CYP11A1, cytochrome P450 family 11 subfamily A member 1; E, enlarged nuclei; CTL, control.

    Journal: Molecular Medicine Reports

    Article Title: Indium (III) chloride inhibits testicular Leydig cell proliferation by disrupting centrosome copy numbers

    doi: 10.3892/mmr.2026.13891

    Figure Lengend Snippet: Genomic instability is observed in InCl 3 -treated TM3 cells. InCl 3 induces enlarged nuclei and micronuclei (as indicated by white arrowheads) in TM3 cells. (A) Nuclear shapes of CTL and InCl 3 -treated TM3 cells were analyzed using DAPI staining. The right panel represents enlarged views of selected regions. Micronuclei, small DAPI staining near the nucleus, are indicated by the arrowheads. Mitotic cells were identified and are marked with red circles. Scale bar, 10 µm. (B) Quantitative results of the nuclear area shown through DAPI staining. (C) Quantification of the proportions of cells with micronuclei [as indicated by selected regions 1 and 2 of (A), right panel]. (D) InCl 3 treatment did not affect the mitotic index. The proportions of mitotic cells (mitotic index) were quantified. (E) InCl 3 treatment reduced the levels of testosterone in the culture medium of TM3 cells. Quantitative results of relative testosterone levels measured through ELISA in the absence or presence of InCl 3 . InCl 3 treatment reduced steroidogenic gene expression. (F) Schematic diagram of testosterone production. Quantitative results of relative mRNA levels of (G) StAR, (H) HSD3B and (I) CYP11A1. Results are presented as mean ± SD from ≥3 independent experiments. *P<0.05, **P<0.01 and ***P<0.001. n.s., not significant; InCl 3 , indium chloride; StAR, steroidogenic acute regulatory protein; HSD3B, 3-β-hydroxy-δ-steroid dehydrogenase; CYP11A1, cytochrome P450 family 11 subfamily A member 1; E, enlarged nuclei; CTL, control.

    Article Snippet: Mouse serum testosterone levels were quantified using a commercially available Mouse T Testosterone ELISA kit (cat. no. EM1850-HS; Wuhan Fine Biotech Co., Ltd.), according to the manufacturer's instructions.

    Techniques: Staining, Enzyme-linked Immunosorbent Assay, Gene Expression, Control